Further Characterization of Alanine Aminotransferase of Rat Liver
نویسندگان
چکیده
منابع مشابه
Alanine Aminotransferase
An examination of the subcellular distribution of alanine aminotransferase activity in pig cardiac tissue showed that about 10% of the total activity was bound to particulate material, with the highest specific activity in the sarcosomal fraction. The soluble enzyme was obtained in a high state of purity, as indicated by sedimentation velocity, starch gel electrophoresis, and spectral analyses....
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L-Alanine:glyoxylate aminotransferase was isolated from human liver and purified 900-fold. Pyridoxal phosphate was required for catalytic activity and enhanced the stability of the enzyme during purification and storage. The enzyme underwent activation when heated for 10 min in 10 PM pyridoxal phosphate. Activation resulted in the formation of a nondialyzable pyridoxal phosphate complex with th...
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Conditions for accurate measurement of catalytic activity of aspartate aminotransferase and alanine aminotransferase in human serum have been reinvestigated. The basic variables (kind of buffer, buffer concentration, pH, ion effects, and the influence of pyridoxal-5-phosphate) can now be considered optimized. On this basis, the kinetic parameters of both aminotransferases were determined, i.e.,...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95965-x